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Caspase 3/7

Caspase-Glo® 3/7 Assay Protocol - Promeg

Diese wiederum spalten die pro -Form (Vorläuferform) nachgeschalteter Caspasen (Effektor -Caspasen, unter anderem Caspase 3, 7, 6), die zelleigene Proteine wie unter anderem Aktin und Lamin spalten Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9.It is encoded by the CASP3 gene.CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts.. The CASP3 protein is a member of the cysteine-aspartic acid protease family

CellEvent™ Caspase-3/7 Green Detection Reagent ist ein zellpermeantes Reagenz, das aus einem Vier-Aminosäurepeptid (DEVD) besteht, welches mit einem nukleinsäurebindenden Farbstoff konjugiert ist As mentioned earlier, a study of 1028 endometrial cancer patients and 1003 healthy controls examined potential links between caspase-3, -7, and -8 variant alleles and risk of endometrial cancer. Of 35 selected SNPs, four in CASP7 were in high linkage disequilibrium and associated with increased risk of endometrial cancer; two CASP7 SNPs were associated with reduced risk; and two CASP7 SNPs were associated with increased risk compared with individuals homozygous for the major CASP7 alleles.

Caspase-3/7 Inhibitor I|Caspase-3/7 inhibitor|CAS# 220509-74-0

Liens accessibles depuis GeneCards et HUGO. Caspase 3 La caspase 3 est une protéase à cystéine de la famille des caspases (de l'anglais cysteine-dependent aspartate-directed proteases, qui interagit avec la caspase 8 et la caspase 9. Elle est codée chez l' homme par le gène CASP3, situé sur les chromosome 4 The Incucyte® Caspase-3/7 Red Dye is specially formulated for use in the Incucyte® Live-Cell Analysis System configured with a Green/Red Optical Module and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis Members of the caspase family of cysteine proteases play central roles in coordinating the stereotypical events that occur during apoptosis. Because the major executioner caspases, caspase-3 and caspase-7, exhibit almost indistinguishable activity toward certain synthetic peptide substrates, this has led to the widespread view that these proteases occupy functionally redundant roles within the. CellEvent™ Caspase-3/7 grünes Detektionsreagenz ist ein neuartiges fluorogenes Substrat für aktivierte Caspase-3/7 für die Bildgebung von lebenden und fixierten Zellen mit Anregungs-/Emissionsmaxima von ca. 502/530 nm

CellEvent™ Caspase-3/7 Green Detection Reagen

ICT's FLICA assay kits are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells and tissues. The FAM FLICA Caspase-3/7 assay probe allows researchers to assess caspase 3 and 7 activation. The FLICA reagent FAM-DEVD-FMK enters each cell and irreversibly binds to active caspases 3 and 7 The Caspase-3/7 Inhibitor I, also referenced under CAS 220509-74-0, controls the biological activity of Caspase-3/7. This small molecule/inhibitor is primarily used for Cancer applications

Caspase-Glo® 3/7 3D Assay - Promega Corporatio

  1. The CaspaTag Caspase-3/7 In Situ Assay Kit, Fluorescein for Flow Cytometry is a fluorescent-based assay for detection of active caspase-3 or caspase-7 in cells undergoing apoptosis. SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente
  2. ex EMEA/Indi
  3. Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular structures such as DNA fragmentation or degradation of cytoskeletal proteins (1). The activity of caspase-3 is tightly regulated and it is produced as.
  4. The FLICA 660 Caspase 3/7 probe allows researchers to assess caspase 3 & 7 activation. The FLICA reagent 660-DEVD-FMK enters each cell and irreversibly binds to activated caspase 3 and 7. Because the 660-DEVD-FMK FLICA reagent becomes covalently coupled to the active enzyme, it is retained within the cell, while any unbound 660-DEVD-FMK FLICA reagent diffuses out of the cell and is washed away.

Caspase 3/7 Assay Kit (Magic Red) (ab270771) Abca

  1. FlowCellect™ Human T Cell Caspase 3/7 Kit This FlowCellectT Human T cell Caspase 3/7 Kit for Flow Cytometry provides a simplified & rapid method to assess apoptosis based on Caspase 3/7 activity in sub-populations of T cells. - Find MSDS or SDS, a COA, data sheets and more information
  2. MCF-7 breast carcinoma cells do not express caspase-3. MCF-7 breast carcinoma cells do not express caspase-3. MCF-7 breast carcinoma cells do not express caspase-3 Breast Cancer Res Treat. 2009 Sep;117(1):219-21. doi: 10.1007/s10549-008-0217-9. Epub 2008 Oct 14..
  3. escent assay that measures caspase-3 and -7 activities. These members of the cysteine aspartic acid-specific protease (caspase) family play.
  4. Interestingly, deficiency of caspase-1/11, the key executioner of pyroptosis, aggravated disease severity caused by ERA virus, whereas wild-type mice or mice deficient for either caspase-3, -7, or both IL-1β and IL-18 presented the typical mild symptoms associated with ERA virus. In conclusion, rabies virus infection of macrophages induces caspase-1- and caspase-3-dependent cell death. In.
  5. Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis | Scientific Reports Apoptosis is one of the most important intracellular events in living cell, which is a..

Added Staurosporine at 3 µM final concentration and Caspase 3/7 substrate at 4 µM final concentration per well and allowed to incubate for 8 hours at 37° C; Imaged the plate every two hours using the Celigo image cytometer for a total of 8 hours; Plate Setup. Results. Drug-treated MDA-MB-231 and Jurkat cells showed an increase in Caspase 3/7 positive cells . Bright field images were. The Muse® Caspase-3/7 Kit allows for the simple, rapid, and quantitative measurement of two important cell health parameters simultaneously Detection of caspase 3/7 Activity in Jurkat cells. Jurkat cells were seeded on the same day at 80,000 cells/well/90 µL in a black wall/clear bottom 96-well costar plate. The cells were treated with or without 20 µM of camptothecin for 5 hours, and/or 5 µM caspase 3/7 inhibitor AC-DEVD-CHO for 10 minutes

The Incucyte® Caspase-3/7 Green Dye is specially formulated for use in the Incucyte® Live-Cell Analysis System and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis Das Nexcelom ViaStainTM Live Caspase 3/7 Detection Kit für 2D/3D Zellkultur detektiert die Aktivität der Caspase 3/7 in lebenden Zellen. Damit lasen sich kinetische Apoptose-Assays mit kontinuierlichem Monitoring dieser Aktivität durchführen. Dieser Test funktioniert frei von Waschschritten und sowohl in 2D- als auch 3D-Zellkulturen Caspases (cysteine-aspartic proteases, cysteine aspartases or cysteine-dependent aspartate-directed proteases) are a family of protease enzymes playing essential roles in programmed cell death. They are named caspases due to their specific cysteine protease activity - a cysteine in its active site nucleophilically attacks and cleaves a target protein only after an aspartic acid residue. As.

Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts After activation by caspase-9, caspase-3 inhibits ROS production and is required for efficient execution of apoptosis, while effector caspase-7 is required for apoptotic cell detachment. Publication types Interestingly, deficiency of caspase-1/11, the key executioner of pyroptosis, aggravated disease severity caused by ERA virus, whereas wild-type mice or mice deficient for either caspase-3, -7, or both IL-1β and IL-18 presented the typical mild symptoms associated with ERA virus. In conclusion, rabies virus infection of macrophages induces caspase-1- and caspase-3-dependent cell death. In vivo caspase-1/11 and caspase-3 differently affect disease development in response to infection with. Cayman's Caspase-3/7 Fluorescence Assay Kit employs a specific substrate, N-Ac-DEVD-N'-MC-R110, which upon cleavage by active caspase-3 or caspase-7, generates a highly fluorescent product that can be measured using excitation and emission wavelengths of 485 and 535 nm, respectively. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds. Caspase-3/7. Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases. Jurkat cells were treated as shown with staurosporine at 1mM to induce apoptosis. The cells were then stained with Caspase 3/7 FAM FLICA™ ( ICT093) and ReadiDrop™™propidium.

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to. Inhibition of caspase 3/7 activity by kaempferol maintains the expression of IAPs in IMR32 cells. The family of cysteine proteases named caspases is critical mediators of apoptosis. Caspases are mainly divided into initiator caspases (caspase-8, -9 and -12) and executioner caspases (caspase-3, -6, and -7)

Caspase-Glo 3/7 3D Reagent was added, mixed briefly, and luminescence measured after a 30-minute incubation at room temperature. Multiplexed cell health data sets can provide a more complete characterization of an apoptotic response at a given endpoint. 0 20,000 40,000 60,000 80,000 100,000 Time (minutes) Luminescence (RLU) 10,0003,33 3nM nM 1,111nM 370nM 123nM 0nM [Panobinostat] 03 06 09 0. The cells were then stained with FLICA 660 Caspase-3/7 Kit (ICT9125) and ReadiDrop propidium iodide (1351101). Apoptotic cells positive for caspases can be seen in the bottom right quadrant and dead cells positive for both caspase and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5 Cell Analyze reguliert. Einen der effektivsten Caspase-3/-7 und -9 Inhibitoren stellt XIAP dar. XIAP besteht aus drei BIR-Domänen und einer RING-Domäne, die über Linker-Sequenzen verbunden sind. Für dieFähigkeit dieexecutioner Caspasen-3 und-7 zu inhibieren, wird ein Teil der BIR1 -BIR2 Linker-Sequenz (im Folgenden alsLinker bezeichnet) und die zweit The Apo-ONE® Homogeneous Caspase-3/7 Assay provides the necessary reagents for fast and sensitive measurement of active caspase-3 and -7 in a homogeneous format

β-elemene induces glioma cell apoptosis by downregulating

Caspase-3, caspase-6, and caspase-7 are effector caspases that execute apoptosis. Activated caspase-3 and caspase-7 can cleave multiple structural and regulatory proteins, which are critical for cell survival and maintenance (Boyce et al., 2004; Crawford & Wells, 2011) Cell Meter™ Caspase Activity Apoptosis Assay Kitsare designed to monitor cell apoptosis through measuring Caspase activation. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak. Learn more >> AAT Bioquest. Contact us. Order info. Quick order. Company Telephone: Fax: Hours: Monday to Friday 8:30 - 17:30 PST (GMT-8) Location: 520 Mercury. Caspase-3/7 Inhibitor I. 220509-74-. CHEMBL60190. 5-[(S)-(+)-2-(Methoxymethyl)pyrrolidino]sulfonylisatin. Isatin Sulfonamide 16. More... Molecular Weight: 324.35 g/mol. Dates: Modify . 2020-12-27. Create . 2006-10-25. Contents. 1 Structures Expand this section. 2 Names and Identifiers Expand this section. 3 Chemical and Physical Properties Expand this section. 4 Related Records Expand this. Cayman's Caspase-3/7 Fluorescence Assay Kit employs a specific substrate, N-Ac-DEVD-N'-MC-R110, which upon cleavage by active caspase-3 or caspase-7, generates a highly fluorescent product that can be measured using excitation and emission wavelengths of 485 and 535 nm, respectively. The kit is simple to use and can be easily adapted to high-throughput screening for therapeutic compounds. The Caspase-Glo ® 3/7 assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo ® 3/7 Reagent in an add-mix-measure format results in cell lysis, followed by caspase cleavage of the substrate

Zum Auslösen des Zelltods werden Initiator-Caspasen (z. B. Caspase-8 und 9) aktiviert. Diese wiederum spalten die pro-(Vorläufer-) Form nachgeschalteter Caspasen (unter anderem Caspase 3, 7 6), wodurch diese aktiviert werden und schließlich zum Zelltod führen. Eine Fehlsteuerung dieser Mechanismen kann zu schweren Krankheiten führen. Ein. Caspase-3/7 signal in apoptotic cells is short lived, requiring researchers to perform multiple replicates of the same experiment to accurately determine if and when apoptosis occurs. A cell line treated with various concentrations of compound can exhibit a relatively short time period in which an apoptosis signal is detected . For example, cells treated with bortezomib showed little caspase.

Caspase 3/7, 8 & 9 Apoptosis Assay using the NC-3000

Caspase-3/7 Green Detection Reagent is 502/530 nm (Figure 1, page 1). Kinetic assay 1. Dilute the CellEvent™ Caspase-3/7 Green Detection Reagent into complete medium to a final concentration of 2-10 μM. Note: We recommend initial testing with 2-10 μM of CellEvent™ Caspase-3/7 Green Detection Reagent. However, the optimal concentration may vary depending on th Caspase-3是一种蛋白酶,1994年Fernandez-Alnemri等在BenBank表达序列标记 (expression sequence tag, EST)数据库中找到一段与ICE/CED-3活性中心同源的序列,用它合成探针后,筛选人Jurkat T淋巴细胞cDNA文库,从中克隆到一种新基因,因其编码分子量为32kD的半胱氨酸蛋白酶而称之为CPP32 (cysteine protease protein, 32kD)。. 随后,其他学者独立地将这一蛋白基因克隆出来,并分别命名为prICE、apopain (凋亡. • The Muse™ Caspase-3/7 reagent is ce ll membrane permeable and non-toxic to the cell. The Muse™ Caspase-3/7 reagent contains a DNA binding dye that is linked to a DEVD peptide substrate. When bound to DEVD the dye is unable to bind DNA. Cleavage by active Caspase-3/7 in the cell results in release of the dye, translocation to th The Caspase-Glo® 3/7 3D Assay is a homogeneous, luminescent assay that measures caspase-3 and -7 activities present in apoptotic 3D cultures. The assay has been validated for use in a wide range of 3D cell models, and is suitable for high-throughput applications. It can also be multiplexed with other homogeneous assays so you can get more data from a single well The Caspase-Glo® 3/7 Assay is ideal for use with multiwell-plate formats as the procedure involves the addition of a single reagent directly to cells cultured in their regular serum-containing media. No further steps are required, such as cell washing or removal of media. Addition of the single reagent lyses the cells and results in the generation of a luminescent signal which is proportional.

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Ki

If caspase-3/7 enzymes (DEVDases) are active, they will cleave the intact (quenched) substrate and release the cresyl violet fluorophore, which will greatly enhance the cresyl violet fluorescence potential. The red fluorescent product will often aggregate inside lysosomes; caspases are not lysosomal enzymes. As protease activity progresses and more substrate is cleaved, the red fluorescent. EarlyTox™ Caspase-3/7 R110 Assay-Kit. Dieses Kit ermöglicht die Analyse der Apoptose auf Grundlage der Caspase-3/7-Aktivität und ihrer Konsens-Targetsequenz Asp-Glu-Val-Asp (DEVD). Caspase-3 und Caspase-7 sind Proteasen, die während der Ausführungsphase von Apoptose aktiviert werden. Dieses Kit ermöglicht die Durchführung eines homogenen Ein-Schritt-Assays, der speziell für. MCF-7, a breast cancer-derived cell line, is deficient of caspase 3 and relatively insensitive to many chemotherapeutic agents. To study the association of caspase 3 deficiency and chemotherapeutic resistance, we reconstituted caspase 3 in MCF-7 cells and characterized their apoptotic response to doxorubicin and etoposide. Western blots demonstrated that caspase 3 was constitutively expressed.

Thus, NucView caspase-3 substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® caspase-3 substrates can be used to detect caspase-3/7 activity within individual intact cells without. The most prominent IAP XIAP inhibits caspase-3, -7 and -9. It interacts with the N-terminal of the small caspase subunit and shields the catalytic side of caspase-3 and -7 by reverse binding (Eckelman,2006). Two other natural, viral pan-caspase inhibitors are known: p35 (Xu,2001) and CrmA (Renatus,2000). 16 selected references: 9 GO-Terms:) ) ) ) Cytosol (also annotated in these classes. The Caspase-3/7 Red assay reagent is specially formulated for use in the Incucyte ® live-cell analysis system and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis EarlyTox™ Caspase-3/7 R110 Assay Kit enables analysis of apoptosis based on caspase-3/7 activity and its consensus target sequence, Asp-Glu-Val-Asp (DEVD). Caspase-3 and caspase-7 are proteases that are activated during the execution phase of apoptosis. This kit provides a single-step, homogenous assay that is specifically designed for microplate readers. The fluorogenic substrate (Ac-DEVD)2.

Caspase 3/7, 8 & 9 Apoptose-Assay mit dem NucleoCounter

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (3-6). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates, such as PARP (3,5). During apoptosis, caspase-7 is activated by upstream caspases through proteolytic processsing at Asp23, Asp198, and Asp206, thereby producing the mature. 兼容多种检测方法:荧光显微镜、流式细胞仪、激光共聚焦及高内涵显微镜检测 支持多重荧光检测: Caspase-3/7 绿色荧光、 PI 红色荧光、 Hoechst33342 蓝色荧光 结果准确可靠:在多种细胞系中进行了有效验证,重复性好、灵敏度高 操作简便快速:仅需两步染色反应, 1 小时即可完成凋亡细胞检

Caspase全称为含半胱氨酸的天冬氨酸蛋白水解酶(cysteinyl aspartate specific proteinase)。caspase是一组存在于细胞质中具有类似结构的蛋白酶。Caspase与真核细胞凋亡密切相关,并参与细胞的生长、分化与凋亡调节 CaspaTag Caspase 3,7 In Situ Assay Kit, Fluorescein: Overview: Apoptosis is an evolutionarily conserved form of cell suicide, which follows a specialized cellular process. The central component of this process is a cascade of proteolytic enzymes called caspases. These enzymes participate in a series of reactions that are triggered in response to pro-apoptotic signals and result in the cleavage. [...] 33258), and apoptosis (caspase 3/7 and 8). dog2008.org Durchflusszytometrisch wurde das Wachstum lebender RPE-Zellen nach 24, 48 und 72 Stunden bestimmt und in Effekte auf Proliferatio Real-time detection of apoptosis in A549 cells treated with cycloheximide alone (control) or TNFα + cycloheximide (test)

Caspase-3/7 Inhibitor I has also been reported to inhibit apoptosis in chondrocytes (44% inhibition at 10 μM and 98% inhibition at 50 μM). Selectivity for caspases 3 and 7 involves unique hydrophobic residues in the S2 pocket surrounding the catalytic cysteine residue. References. 1. Lee, D., et al. 2000. J. Biol. Chem. 275: 16007-16014. PMID: 10821855 2. Lee, D., et al. 2001. J. Med. Chem. CaspaTag Caspase 3,7 In Situ Assay Kit, Fluorescein The CaspaTag Caspase-3/7 In Situ Assay Kit, Fluorescein for Flow Cytometry is a fluorescent-based assay for detection of active caspase-3 or caspase-7 in cells undergoing apoptosis.; find Sigma-Aldrich-APT423 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich The Caspase-Glo 3/7 Assay provides a homogeneous luminescent assay that measures caspase-3/7 activities. The assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo 3/7 Reagent in an add-mix-measure format results in cell. Apoptose-Inhibitoren (englisch Inhibitors of apoptosis protein, IAP) sind eine Familie von Proteinen, die erstmals bei Baculoviren entdeckt wurden und als endogene Inhibitoren für die Apoptose (programmierter Zelltod) wirken.. Diese Seite wurde zuletzt am 4. Januar 2020 um 18:44 Uhr bearbeitet Caspases are a family of cysteine proteases widely known as the principal mediators of the apoptotic cell death response, but considerably less so as the contributors to the regulation of pathways.

Mechanism of Apoptosis with Clinical relevance | Epomedicine

Caspasen - Wikipedi

The Muse Caspase-3/7 assay is versatile and works with both adherent and suspension cells and can be used with a variety of inducers to provide a measure of apoptotic populations. The Muse Caspase-3/7 Assay provides accurate percentages of caspase-activ e cell populations compared to data obtained using comparative platforms Caspase 3/7 is also identified as a drug-screening target. Caspase inhibitors have anti-cancer and other pharmalogical potentials. It has been proven that Caspase 3/7 has substrate selectivity for the peptide sequence Asp-Glu-Val-Asp (DEVD). The Caspase 3/Caspase 7 Assay Kit (Green) uses (Z-DEVD)2 R110 as fluorogenic indicator for assayin

Caspase 3 - Wikipedi

Here, we report a highly functionalized nanoprobe, FLAME-DEVD 2, with an OFF/ON 19F MRI switch for detecting caspase-3/7 activity based on the paramagnetic relaxation enhancement effect. To improve the cleavage efficiency of peptides by caspase-3, we designed a novel Gd3+ complex-conjugated peptide, DEVD X (X = 1, 2), which is a substrate peptide sequence tandemly repeated X times, and demonstrated that DEVD 2 showed faster cleavage kinetics than DEVD 1. By incorporating this novel concept. The total number of apoptotic cells was determined by counting the cells stained with green Caspase 3/7 reagent Plate-Level View allows for quick observations of the total number of green Caspase 3/7 positive cells. Shown below are typical results of apoptotic (Caspase 3/7 positive) cells after 8 hours of drug treatment

Molecular Probes™ CellEvent™ Caspase-3/7 Green Flow

Diese wiederum spalten die pro - (Vorläufer-) Form nachgeschalteter Effektor-Caspasen (unter anderem Caspase 3, 7, 6), die zelleigene Proteine wie unter anderem Aktin und Lamin spalten. Eine weitere wichtige Funktion der Effektor-Caspasen ist die Aktivierung einer Nuklease, die im Rahmen der Apoptose die nukleäre DNA spaltet The Incucyte® Caspase-3/7 Dyes are inert, non-fluorescent (DEVD) substrates that freely cross the cell membrane where they can be cleaved by activated caspase-3/7 to release either a green or red DNA-binding fluorescent label. Apoptotic cells are identified by the appearance of fluorescently-labeled nuclei Caspase-3/7-mediated Cleavage of β2-spectrin is Required for Acetaminophen-induced Liver Damage . Hye Jung Baek 1, Yong Min Lee 1, Tae Hyun Kim 1, Joo-Young Kim 1, Eun Jung Park 2, Kuniyoshi Iwabuchi 3, Lopa Mishra 4, Sang Soo Kim 1 . 1. Radiation Medicine Branch, and 2. Cancer Immunology Branch, National Cancer Center, Goyang, Gyeonggi, 410-769, Korea, 3. Department of Biochemistry I, School. The Caspase-Glo® 3/7 Assay is a luminescent assay that measures caspase-3 and -7 activities in purified enzyme preparations or cultures of adherent or suspension cells. The assay provides a proluminescent caspase-3/7 substrate, which contains the tetrapeptide sequence DEVD. This substrate is cleaved to release aminoluciferin, a substrate of luciferase used in the production of light. The.

The delay we observe between the average times of Ca 2+ peak and onset of caspase-3/7 activation , as well as the variation in the time interval between Ca 2+ peak and onset of caspase-3/7 activation are thus manifestations of the expected cumulative effect of fluctuations in the times of the many biochemical reactions involved between the cytochrome c release, apoptosome formation, and the caspase-3/7 activation Caspase-Glo™ 3/7 Assay is a homogeneous luminescent assay that measures caspase-3/7 activities $173.12 - $4100.00 See promotional offers below. Specifications. For Use With (Application) 96- or 384-well plates using either purified Enzyme or cells in culture as sample : Storage Requirements −30 to −10°C: View More Specs. Includes: Caspase-Glo 3/7 Substrate; Caspase-Glo 3/7 Buffer. Importantly, targeting caspase-1 either genetically or pharmacologically reduced caspase-3/7 activation, suggesting that caspase-1 is upstream of caspase-3/7 activation and offering a therapeutic opportunity for caspase-3/7 inhibition. Taken together, our findings support the hypothesis that pyroptotic stimuli can activate a network of caspases, including caspase-1, -3, and -7, all of which. Research Proven easy to use FLICA™ Caspase 3&7 Assay Kit, green. This in vitro apoptosis kit make it easy to measure cell death and apoptosis in cultured neurons and many other cell types. Just add the reagent to the media, let it incubate 1- 4 hours, wash the cells and read with a fluorescence plate reader, microscope, or flow cytometer. Apoptotic cells fluoresce green CAS: 220509-74- MDL: MFCD22581511 A potent, cell-permeable, reversible inhibitor of caspase-3

Caspase Functions in Cell Death and Diseas

  1. Caspases are a family of proteases that initiate the process of apoptosis in mammalian cells. Both Caspase-3 and 7 are the members of cysteine aspartate proteases. Caspase-3, also referred as CPP32, is a member of the CED-3 subfamily of caspases and most studied caspases of the mammalian cells
  2. After activation by caspase-9, caspase-3 inhibits ROS production and is required for efficient execution of apoptosis, while effector caspase-7 is required for apoptotic cell detachment
  3. The Caspase-3/7 assay reagent is specially formulated for use in the IncuCyte® live-cell analysis system and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis
  4. Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3.

Caspase 3 — Wikipédi

  1. Caspase-3/7 Staining Kit provides a convenient means for detecting activated caspase-3/7 in living cells. The assay utilizes the caspase inhibitor DEVD-FMK conjugated to FITC (Green-DEVD-FMK) as the fluorescent in situ marker. Green-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to the activated caspase-3/7 in apoptotic cells
  2. Caspase-3/7 inbibitor I is a potent, reversible, isatin sulfonamide-based inhibitor of caspase-3 (KI (app) = 60 nM) and caspase-7 (KI (app) = 170 nM). Is a weaker inhibitor of caspase-9 (Ki (app) = 3.1 mM). It Has only a trivial effect (Ki (app) >25 mM) on the activities of caspase-1, caspase-2, caspase-4, caspase-6, and caspase-8
  3. When caspase 3-7 is activated, link is cleavaged and FP602 change its localization appearing in the nucleus. This cell line allows to monitor apoptosis by caspase 3-7 activation, quantifying the red fluorecence intensity inside the cell nucleus. Each vial of Caspase 3-7 Activity Assay Cell Line cell line contains 3 million cells. It is guaranteed that these cells will expand following the cell.
  4. The Caspase-3/7 for Metabolism Dye is specially formulated for use in the Incucyte® Live-Cell Analysis System, configured with the SX5 Metabolism Optical Module, and can be added directly to tissue culture wells using a no wash, mix and read protocol to acquire live cell images of cells undergoing caspase-3/7 mediated apoptosis
  5. escent Caspase-Glo 3/7.
  6. Effektor-Caspasen (z. B. humane Caspase-3, -7) haben eine kurze oder keine Prodomäne. Sie werden meist durch Initiator-Caspasen während einer späteren Phase der Apoptose aktiviert. Deren Aktivität führt zum typischen, apoptotischen Phänotyp. Substrate dieser Caspasen sind zelluläre Proteine, die an der Regulation des Zellzyklus, an der DNA- Reparatur oder am Erhalt der Zellstruktur.

Caspase-3/7 activation in DU145 cells treated with docetaxel, cisplatin, or TNF-α . It is well known that caspases play important roles in apoptotic cell death. Therefore, we measured activities of executioner caspase-3/7 in DU145 and LNCaP cells, the latter of which are known to undergo apoptosis in response to docetaxel . In the case of DU145-cont cells, docetaxel and cisplatin slightly. NucView® caspase-3 substrates are based on novel fluorogenic DNA dyes that have been coupled to the caspase-3/7 recognition sequence (DEVD). When introduced to cells, the substrate is initially non-fluorescent and is able to penetrate the plasma membrane and enter the cytoplasm. During apoptosis, caspase-3/7 cleaves the substrate and releases the high-affinity DNA dye which leads to nuclear. A caspase 3 é um das caspases existentes. Interage com a caspase 8. O gene correspondente codifica uma proteína que é membro da família das proteases de cisteína-aspartato. A activação sequêncial das caspases desempenha um papel central na fase de execução da apoptose celular In addition, the Incucyte® Caspase-3/7 Green Dye is non-perturbing to cell growth and morphology. When added to tissue culture medium, this inert, non- fluorescent substrate crosses the cell membrane where it is cleaved by activated caspase-3/7 resulting in the release of the DNA dye and green fluorescent staining of nuclear DNA. Kinetic. カスパーゼ-3(英: caspase-3 )は、カスパーゼ-8、 カスパーゼ-9 (英語版) と相互作用するカスパーゼであり、ヒトではCASP3遺伝子にコードされる。 CASP3のオルソログ は、全ゲノムデータが利用可能な哺乳類の多数で同定されている。 オルソログは、鳥類、爬虫類、両生類、真骨類にも存在して.

Incucyte® Caspase-3/7 Red Dye for Apoptosis - Incucyte eSho

  1. La caspase 3 est une protéase à cystéine de la famille des caspases [3] (de l'anglais cysteine-dependent aspartate-directed proteases, qui interagit avec la caspase 8 et la caspase 9. Elle est codée chez l'homme par le gène CASP3, situé sur les chromosome 4. Des orthologues du gène CASP3 ont été identifiés chez un grand nombre de mammifères pour lesquels des données génomiques.
  2. Caspase 3/7 activity was measured using Caspase-Glo 3/7 assay kit (Promega, USA).. For testing reference compounds, on d 6 of the differentiation, cells were co-treated with 80 μmol/L MPP+ together with various concentration of compound SB216763 (Sigma, USA) or compound GW8510 (Sigma, USA) or their vehicle for 2 d. ATP levels and caspase 3/7 activity were measured. Article Title: Bile acids.
  3. ating unwanted cells during embryonic development, growth, differentia- tion, and maintenance of tissue.
  4. CASP7 (Caspase 7) is a Protein Coding gene. Diseases associated with CASP7 include Cardiomyopathy, Familial Hypertrophic, 1 and Legionellosis.Among its related pathways are Apoptosis Modulation and Signaling and ERK Pathway in Huntingtons Disease.Gene Ontology (GO) annotations related to this gene include cysteine-type endopeptidase activity and aspartic-type endopeptidase activity
  5. caspase-3的原结构域明显短于ICE,但蛋白酶活性中心和与结合底物有关的保守的氨基酸均与ICE一致。pro-caspase-3在活化过程中从Asp28~Ser29和Asp175~Ser176两处被剪切,形成P17(29~175)和P10(182~277)两个片段,相当于ICE的P20和P10,两种亚基再组成活性形式的caspase-3。pro-caspase-3.
  6. e 110.
Ursolic acid inhibits growth and induces apoptosis inThe common anesthetic, sevoflurane, induces apoptosis in

Executioner caspase-3 and caspase-7 are functionally

Caspase-Aktivität und programmierter Zelltod in Neuroblastomzellen: Regulation durch das Tumorsuppressorprotein p53 Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften der Medizinischen Fakultät der UNIVERSITÄT DES SAARLANDES 2012 vorgelegt von: Anja Keim geb. am: 3.05.1983 in Kaiserslautern . Erklärung Ich erkläre hiermit, dass ich die vorliegende Arbeit ohne. SensoLyte Homogeneous AMC Caspase - 3/7 Assay Kit Catalog number AS-71118 (1kit) Description Fluorimetric Source AnaSpec Product category Assays & Kits Product sub category Functional Assays Application Fluorescence Shipment info Cool Pack Nacres Codification NA.84. Request the Datasheet. First name . Last name . Email * Country *: Company name * Fields marked with an asterisk are required.

Invitrogen™ CellEvent™ Caspase-3/7 Green Detection Reagent

The EarlyTox ™ Caspase-3/7 NucView ™ 488 Assay Kits enable detection of apoptosis in intact cell populations through use of NucView 488 Caspase-3 substrate. This substrate consists of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. Initially non-fluorescent, it permeates the cell membrane, and if the cell is apoptotic, the substrate is cleaved by caspase-3/7. Promega caspase 3 7 assayactivation Caspase 3 7 Assayactivation, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and mor

The cGAS-cGAMP-STING Pathway: A Molecular Link Between

Caspase 3 and 7 Assay to detect caspase activit

Optimiertes Caspase-3/7-Substrat für die apoptotische Analyse; Einfaches und schnelles waschfreies Protokoll schont die empfindlichen apoptotischen Zellen; Kompatibel mit der Fluoreszenzbildgebung lebender Zellen und mit Formaldehyd-basierten Fixierverfahren; Gebrauchsfertige flüssige Zubereitung: kein Verdünnen, Wiegen oder Pipettieren ; Peptid mit vier Aminosäuren (DEVD), konjugiert an. Information on EC 3.4.22.56 - caspase-3. strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-/- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this positio Livin has direct interaction with several caspases including caspase-3, -7, and -9. The direct activation of executioner caspases is an anticancer strategy that may prove beneficial in treating the many cancers in which procaspase-3 concentrations are elevated. (prostatakrebs-bps.de

Drug Resistance and Molecular Cancer Therapy: Apoptosis
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